2nd World Congress Virology and Infectious disease
Date: September 3-4, 2019
Venue: London, UK
A New Generation of Animal Cell Expression
We have developed a completely unique DNA expression system, based on the Semliki Forest virus (SFV) replicon, which combines a good selection of animal cell hosts, high potency and easy use.
DNA of interest is cloned into SFV inclusion body vectors that function templates for in biosynthesis of the recombinant polymer.
The polymer is transfected with virtually 100 per cent potency into animal tissue culture cells by suggests that of electroporation.
Among the cell, the recombinant polymer drives its own replication and capping and ends up in a large production of the heterologous macromolecule whereas competitory out the host macromolecule synthesis.
The expression system conjointly includes an in vivo packaging procedure whereby recombinant ribonucleic acid is packaged into infectious virus particles victimization cotransfection with packaging–deficient helper polymer molecules.
The ensuing high concentration recombinant virus stock is used to infect a good varies of animal cells with ulterior high expression of the heterologous factor product, however blankly of any structural proteins of the helper.
The infected cells turn out macromolecule for up to seventy-five hours post-infection when the heterologous product will represent the maximum amount as twenty-fifth of the overall cell macromolecule.
The final utility of the system is incontestible through the expression of the human siderophilin receptor, mouse dihydrofolate enzyme, chick muramidase and Escherichia coli β–galactosidase.
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Contact details
Clara Charlotte
Program Manager | Virology 2019
Email:virology@microbioconferences.com
Clara Charlotte
Program Manager | Virology 2019
Email:virology@microbioconferences.com
Phone: +44 20 3769 1755
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